Molecular detection and identification of Rickettsia felis in Polygenis (Siphonaptera, Rhopalopsyllidae, Rhopalopsyllinae) associated with cricetid rodents in a rural area from central Argentina

Abstract

The aim of this study was to detect and identify the presence of Rickettsia in fleas associated with cricetid rodents from northeastern Buenos Aires Province, Argentina. Sixteen fleas belonging to three species of Polygenis were collected from 56 cricetid rodents and analyzed for the presence of Rickettsia performing the conventional polymerase chain reaction (PCR) technique. Only one specimen of Polygenis (Polygenis) axius axius collected from Oxymycterus rufus was positive for Rickettsia felis using the gltA gene, and to ompA gene. This is the first report of R. felis in a Rhopalopsyllidae flea from Argentina, and the first detection of this bacterium in P. (P.) a. axius. Since both, O. rufus and P. (P.) a. axius, are common in areas close to humans, and enzootic cycle of R. felis is not fully understood, the results herein obtained might be of epidemiological importance. Further studies are needed in order to analyze the capacity of the species of Polygenis to transmit R. felis.