The port of Buenos Aires is located in the Rio de la Plata river on the riverside of the homonymous city. The fish community that inhabits the port water’s was studied in order to know the specific composition, relative abundance, spatial distribution and its relationships with the port activities. Ten sampling places were considered, seven inside the port and three in the access channels. The sampling was realised on August and September of 1998, using active and passive fishing gears. Diversity indices, Margalef' (Rl) and Shannon and Weaver (HO, were calculated. A cluster analysis was carried out with relative abundance of each taxa. In access channels, four species were captured and its relative abundance and diversity were similar for each stations. Contrarily inside the port twenty-seven species was registered, the relative abundance and specific composition were more fluctuate than out side. The heterogeneity observed in the distribution of the fish community would be linked to the design of the port and the activities that are developed in each place.