Knowledge about ictiofaunal spatial distribution and composition of the lotic enviroment pampean plain is scarce. The purpose of this paper is to characterize fish community living in the Rodríguez Stream, located in the urban northeastern area of the province of Buenos Aires in Argentina. Limnological data was collected and analyzed along the stream. Ichthyological samples were taken using different methods to determine the quali-quantitative composition of the fish and their changes tendencie to change in relation to related to some diferent variables. The low oxygen concentration in the middle course caused by the highest anthropic pressure, was the key factor wich was responsible for the largest changes in the composition and species abundance of the fauna. This contribution, besides considering biological aspects of the ichthyofauna of this stream for the first time provides a diagnosis how this is affected by the growing urban impact within the metropolitan area of Buenos Aires.