Production of rutin and Kaempferol-3-O-glucuronide by tissue cultures of Alpinia purpurata (Vieill) K. Schum

Abstract

The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata through HPLC analysis. Cultures were maintained in liquid MS medium in the following treatments: MS0 (control), TDZ, BAP and IAA + TDZ. Kaempferol-3-O-glucuronide content was higher than rutin for all in vitro treatments, except by control medium in which plants did not produce kaempferol-3-O-glucuronide. A remarkable increase in kaempferol-3-O-glucuronide content was verified using BAP 2 mg.l^-1 (0.027 mg/100 mg dried extract) and IAA 2 mg.l^-1 + TDZ 2 mg.l^-1 (0.030 mg/100 mg dried extract) treatments. With the addition of 2 mg.l^-1 BAP, rutin concentration also increased in the proportion 1:4 compared to control.