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dc.date.accessioned 2012-04-04T14:17:20Z
dc.date.available 2012-04-04T03:00:00Z
dc.date.issued 2012 es
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/8446
dc.description.abstract A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of torsemide in rabbit plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm×150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 349 for torsemide and m/z 326 for the IS. Calibration plots were linear over the range of 5-1000 ng/mL for torsemide in rabbit plasma. Lower limit of quantification (LLOQ) for torsemide was 5 ng/mL. Mean recovery of torsemide from plasma was in the range of 82.7-88.2 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of torsemide in rabbit plasma. es
dc.format.extent 162-166 es
dc.language en es
dc.subject Piridinas es
dc.subject Plasma es
dc.subject Conejos es
dc.subject Espectrometría de Masas es
dc.subject Cromatografía Liquida es
dc.title Quantification of torsemide in rabbit plasma by liquid chromatography/electrospray mass spectrometry and its application es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_2_5.pdf es
sedici.creator.person Hu, Lufeng es
sedici.creator.person Chen, Xiaole es
sedici.creator.person Chen, Chan es
sedici.creator.person Wang, Jinjin es
sedici.creator.person Wang, Zhibin es
sedici.creator.person Wang, Zhiyi es
sedici.subject.materias Farmacia es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001934 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 1 es


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